Journal: Frontiers in Genetics

Article Title: HOTAIR regulates osteogenic–adipogenic differentiation imbalance through the miR-217/DKK1-mediated Wnt/β-catenin pathway in SONFH-BMSCs

doi: 10.3389/fgene.2026.1917162

Figure Lengend Snippet: Primers for qRT-PCR analyses.

Article Snippet: The membranes were blocked with 5% non-fat milk in Tris-buffered saline with 0.1% Tween-20 (TBST) for 1 h at room temperature and then incubated overnight at 4 °C with primary antibodies targeting Osterix (Boster, Cat. No. M02077, 1:1,000), BMP2 (Boster, Cat. No. BM4960, 1:1,000), RUNX2 (Boster, Cat. No. BM4700, 1:1,000), PPARγ (Boster, Cat. No. M00449-2, 1:1,000), C/EBPα (Boster, Cat. No. A00386-1, 1:1,000), Adipsin (Boster, Cat. No. M01134, 1:1,000), DKK1 (Boster, Cat. No. A09360-1, 1:1,000), OCT4 (Abmart, Cat. No. T55781 , 1:10,000), β-catenin (Abmart, Cat. No. M24002 , 1:10,000), and β-actin (Boster, Cat. No. BM3873, 1:10,000).

Techniques: Sequencing

Journal: Frontiers in Genetics

Article Title: HOTAIR regulates osteogenic–adipogenic differentiation imbalance through the miR-217/DKK1-mediated Wnt/β-catenin pathway in SONFH-BMSCs

doi: 10.3389/fgene.2026.1917162

Figure Lengend Snippet: HOTAIR silencing inhibits adipogenic differentiation of SONFH-BMSCs in vitro . (A) Oil Red O staining of FNF-derived control BMSCs (NC), untreated SONFH-BMSCs, SONFH si-NC, and SONFH si-HOTAIR groups at days 7 and 14. (B) Quantitative analysis of Oil Red O staining at days 7 and 14. (C) qRT-PCR analysis of PPARγ, C/EBPα, and Adipsin mRNA levels. Expression was normalized to GAPDH and is shown compared with untreated SONFH-BMSCs and si-NC controls. (D) Western blot analysis of PPARγ, C/EBPα, and Adipsin. Data are presented as mean ± SD from three independent biological replicates using BMSCs from independent donors, with three technical replicates per experiment. (*P < 0.05; **P < 0.01; ***P < 0.001).

Article Snippet: The membranes were blocked with 5% non-fat milk in Tris-buffered saline with 0.1% Tween-20 (TBST) for 1 h at room temperature and then incubated overnight at 4 °C with primary antibodies targeting Osterix (Boster, Cat. No. M02077, 1:1,000), BMP2 (Boster, Cat. No. BM4960, 1:1,000), RUNX2 (Boster, Cat. No. BM4700, 1:1,000), PPARγ (Boster, Cat. No. M00449-2, 1:1,000), C/EBPα (Boster, Cat. No. A00386-1, 1:1,000), Adipsin (Boster, Cat. No. M01134, 1:1,000), DKK1 (Boster, Cat. No. A09360-1, 1:1,000), OCT4 (Abmart, Cat. No. T55781 , 1:10,000), β-catenin (Abmart, Cat. No. M24002 , 1:10,000), and β-actin (Boster, Cat. No. BM3873, 1:10,000).

Techniques: In Vitro, Staining, Derivative Assay, Control, Analysis, Quantitative RT-PCR, Expressing, Western Blot

Journal: Journal of medicinal chemistry

Article Title: A novel N-substituted valine derivative with unique PPARγ binding properties and biological activities

doi: 10.1021/acs.jmedchem.0c01555

Figure Lengend Snippet: Effect of 7j on PPARγ phosphorylation. (A) Percentage of in vitro PPARγ Ser273 phosphorylation by CDK5 in the presence of 0.1 μM of 7j or Rosi. (B) Phosphorylation of PPARγ Ser273 in 3T3-L1 adipocyte incubated for 60 minutes with 5 μM of Rosi, 7j or Roscovitine (Rsv) before TNFα stimulation (50 ng/ml; 90 minutes). Two independent experiments are shown. Phosphospecific antibodies were from Rockland or New England Peptide (NEP). Arrow heads indicate phosphorylated PPARγ and PPARγ2. (C) RT-PCR analysis of the expression levels of a selection of genes known to be regulated by CDK5-dependent phosphorylation of PPARγ. Values are means ± SD (n = 5) expressed relative to the mean of control. **p < 0.01, ***p < 0.001 vs. control (one-way ANOVA followed by Dunnett’s post hoc test).

Article Snippet: PPARγ phospho Ser273 antibodies were from Rockland (Limerick, PA, USA) or custom produced by New England Peptide (Gardner, MA, USA).

Techniques: Phospho-proteomics, In Vitro, Incubation, Reverse Transcription Polymerase Chain Reaction, Expressing, Selection, Control